Description
E extract with IC50 of 7.6 ?1.5 and 6.04 ?0.5 g/mL respectively, when compared
E extract with IC50 of 7.6 ?1.5 and 6.04 ?0.5 g/mL respectively, when compared with Doxorubicin (DOX) as standard anticancer drug (with IC50 = 0.28, 0.42 and 0.42 g/mL against Hela, HepG2 and MCF-7 respectively) as shown in Table 2. This means that the cytotoxicity pattern of the crude extract on both MCF-7 and HeLa cell lines is similar while differs on HepG2 and EACC (Figure 3). These results indicate that the effect of crude extract on MCF-7 and Hela cells is concentration dependant through the concentrations tested (1-1000 g/mL). In HepG2 cells however,Aboul-Enein et al. BMC Complementary and Alternative Medicine 2014, 14:397 http://www.biomedcentral.com/1472-6882/14/Page 7 ofTable 1 IC50 (g/mL) of different compounds separated from Eichhornia crassipes against DPPH and ABTS radical, and its relative percentage to Ascorbic acid as natural antioxidantMaterials Crude extract Compound A Compound B Compound C Compound D Compound E Compound F Compound G Compound H Compound I *BHT *BHA *Ascorbic acid LSD 0.01 DPPH method ABTS method Relative percentage to Vit. C 74.8 ?4.5f 99.2 ?5.8a 97.1 ?3.b50.8 ?2.4g 77.0 ?3.2c 78.5 ?2.a23.64 15.6 15.29 15.47 16.39 15.84 15.40 15.84 17.20 15.97.4 ?2.7b 92.4 ?6.e77.6 ?1.3b 73.2 ?4.e95.4 PluriSIn 1 ?3.1d 97.2 ?4.b75.8 ?2.6d 77.9 ?1.ab97.2 ?1.6b 96.5 ?2.c75.8 ?1.6d 69.8 ?3.j f97.0 ?5.4b 8.7 ?0.i76.8 ?3.1c 5 ?0.02 9.4 ?0.55i 12.0 ?0.60h 0.11.5 ?0.95h 18.6 ?1.0 g 0.*Standard antioxidants. Each value is represented as mean of triple treatments, means within each row with different letters differ significantly PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28478885 at P < 0.01 according to Duncan's multiple range test.slight toxicity was noticed at low concentrations (below 10 g/mL) after which the effect was very strong hence most of cells were died at about 15 g/mL. This effect can be explained as receptor independent for these type of cells [31,32]. Upon fractionation, compound I showed theTable PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27847310 2 Cytotoxicity [Expressed as IC50 (g/mL)] of crude extract of Eichhornia crassipes and its fractionated products on different tumor cell linesIC50 (g/mL) Materials Crude extract Compound A Compound B Compound C Compound D Compound E Compound F Compound G Compound H Compound I *DOX LSD 0.01 HeLa cell line 1.6 ?0.5j 7.7 ?gHepG2 cell line 7.6 ?1.5i 40.2 ?10.cMCF-7 cell line 1.2 ?0.2i 13.6 ?5.gEACC 6.04 ?0.5j 8.61 ?2.1f 17.3 ?3.5b 7.29 ?1.6h 6.42 ?0.8i 8.19 ?1.2g 9.9 ?2.6e 12.7 ?4.2c 12.3 ?2.7d 22.8 ?6.9a ?0.10.7 ?1.3d 12.8 ?5.b28.3 ?3.7d 74.2 ?12.5 23.6 ?7f 56.1 ?12.b a13.4 ?1.9g 19.4 ?9.2e 27.2 ?2.5d 31.4 ?9.c4.3 ?2.3i 9.9 ?3.e6.9 ?3.1h 9.0 ?3.f14.9 ?8.1h 23.8 ?5.e41.3 ?6.4b 17.5 ?4.f14.1 ?7.0a 11.8 ?7.c15.4 ?3.6g 0.8 ?0.j11.1 ?6.1h 69.1 ?4.a0.28 ?0.07j 0.0.42 ?0.05j 0.0.42 ?0.11j 0.Each value is presented as mean of triple treatments, means within each row with different letters differ significantly at P < 0.01 according to Duncan's multiple range test. *DOX: Doxorubicin (Standard anticancer drug).most potent cytotoxic profile among all other fractions with IC50 of 0.8 ?0.4 g/mL (in HepG2 liver cancer cell line). Compound D showed also high potent cytotoxicity against HeLa cervix cancer cell line (IC50 = 4.3 ?2.3 g/mL) and the other compounds showed moderate cytotoxic effect with IC50's ranging from 7.7 to 14.1 g/mL. With respect to MCF-7 breast cancer cell line, compounds H, B and A showed the best cytotoxic profile with IC50's of 11.1 ?6.1 g/mL, 13.4 ?1.9 g/mL and 13.6 ?5.3 g/mL, respectively. In addition, the other compounds G and C showed mild cytotoxic effects with IC50's rang.